Fig. 5.

Total microglia in white matter. a 10× photomicrographs of representative Iba-1 immunolabelled microglial cells in the corpus callosum, internal capsule and fimbria hippocampi from the boxed regions indicated on the whole brain section insertion, right hemisphere. Photographs of the microglia in the three white matter regions of the 3-month-old TG rat are shown in the right column. Scale bar 100 μm. b Area coverage by a positive signal (as percentage of a total area of a region) for corpus callosum, internal capsule and fimbria. c Iba-1 positive microglia cell counts in a field of area 100 mm² in the in the corpus callosum, internal capsule, and fimbria hippocampi. Animal numbers are as follows: control WT (n = 4), control TG (n = 4), HCD WT (n = 4), HCD TG (n = 4). Values are presented as mean ± SEM. Significance is indicated by * for control WT vs. both TG groups in the corpus callosum (in b, c), HCD TG vs HCD WT in corpus callosum (in c), and control WT vs HCD TG in the internal capsule (in b). One-way ANOVA and Tukey’s multiple comparisons test, p < 0.05. 3M three-month-old TG rat, HCD hypercaloric diet, TG transgenic, WT wildtype