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. 2020 Jan 21;17:29. doi: 10.1186/s12974-020-1698-7

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© The Author(s). 2020

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Synaptic density in the hippocampus identified by synaptophysin (SYN) immunohistochemistry. a 20× photomicrographs of the pyramidal neurons in the dorsal hippocampus CA1 (top row) and CA3 (bottom row) subregions. Scale bar 50 μm. Boxed area corresponds to a field defined for quantification. b Synaptophysin area coverage (%) in CA1 and CA3 regions of the hippocampus. Animal numbers are n = 6 in each group. Values are presented as mean ± SEM. Significance is indicated by * for HCD WT vs both TG groups in CA1 and for both TG groups vs both WT groups in CA3 region. HCD hypercaloric diet, TG transgenic, WT wildtype. One-way ANOVA, Tukey’s multiple comparisons test, p < 0.05