Table 1.
C. glutamicum genes responding to Sec secretion stress in microarray experiments
| Gene locus | Gene | Annotation | Average mRNA ratio ΔSP-AmyE overproductiona |
Average mRNA ratio AmyE overproductionb |
Average mRNA ratio NrpE-cutinase overproductionc |
|---|---|---|---|---|---|
| cg0998 | htrA | HtrA protease homologue | 1.24 | 4.22 | 4.40 |
| cg0706 | esrI | EsrI, inhibitor of the EsrSR two-component system | 0.99* | 2.31 | 0.80* |
| cg1325 | Putative stress responsive transcriptional regulator | 1.13* | 3.69 | 0.96* | |
| cg3264 | rsmP | RsmP, conserved cytoskeletal protein | 0.61 | 2.67 | 0.96* |
Significant (p ≤ 0.05) more than twofold changes in the average mRNA ratios in the DNA microarray experiments are indicated in italic numbers. Average mRNA ratios with p > 0.05 are marked by an asterisk. All microarray data were from three independent biological samples
aAverage mRNA ratio of C. glutamicum (pEKEx2-ΔSP-AmyE) induced/uninduced by IPTG for 30 min
bAverage mRNA ratio of C. glutamicum (pEKEx2-AmyE) induced/uninduced by IPTG for 30 min
cAverage mRNA ratio of C. glutamicum (pEKEx2-NprE-cutinase) induced/uninduced by IPTG for 30 min