Table 3.

Bacterial strains and plasmids used in this study

Strains or plasmids	Relevant properties	Source of references
E. coli strains
DH5α	supE44, ∆lacU169(ϕ80 lacZ∆M15) hsdR17 recA1 endA1 hsdR gyrA relA thi	[53]
C. glutamicum strains
ATCC13032	Wild-type	[54]
ATCC13032 K9	htrA::htrA′-eypf (replacement of htrA by htrA′-eyfp)	This study
C. glutamicum plasmids
pSenLys	Lysine sensor plasmid encoding LysG and its target promoter PlysE fused to eyfp	[44]
pK19mobsacB	Vector for allelic exchange in C. glutamicum, pK18 oriVE.coli, oriT, mob, sacB, lacZα, KmR	[45]
pK19mobsacB-K9	pK19mobsacB containing the eyfp gene flanked by the upstream region and the first 51 bp of the htrA structural gene and the htrA downstream region	This study
pEKEx2	C. glutamicum/E. coli shuttle vector for regulated gene expression, pBL1 oriVC.glutamicum, pUC18 oriVE. coli, Ptac, lacIq, KmR	[55]
pEKEx2-AmyE	pEKEx2 containing a gene encoding B. subtilis AmyE with its authentic signal peptide and a carboxyl-terminal His-tag	This study
pEKEx2-ΔSP-AmyE	pEKEx2 containing a gene encoding a signal peptide-less variant of B. subtilis AmyE with a carboxyl-terminal His-tag	This study
pEKEx2-NprE-cutinase	pEKEx2 containing a gene encoding the mature part of the cutinase from F. solani pisi fused to the NprE signal peptide	[10]
pEKEx2-YpjP-cutinase	pEKEx2 containing a gene encoding the mature part of the cutinase from F. solani pisi fused to the YpjP signal peptide	[10]
pEKEx2-YwmC-cutinase	pEKEx2 containing a gene encoding the mature part of the cutinase from F. solani pisi fused to the YwmC signal peptide	[10]

Km^R kanamycin resistance