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. 2020 Jan 21;20:19. doi: 10.1186/s12890-019-1036-y

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Effects of LPS stimulation with or without Gap19 treatment on apoptosis and ROS levels in HUVEC. After continuous exposure to 400 ng/mL LPS with or without 100 μM Gap19 for 24 h, apoptosis (a, b) and ROS levels (c, d) in HUVECs were assessed, respectively, by Annexin V-FITC/PI and DCFH-DA using flow cytometry. Gap19 was used as an inhibitor of Cx43. Experiments were performed in 3 biological replicates and data are presented as mean ± SD. **P < 0.01 compared with control. ^##P < 0.01 compared with LPS