Figure 2.

Human pluripotent stem cell (hPSC) colonies and hPSC elongate along the grating axis of 2 μm gratings.

(A) 2 μm gratings are reproduced with high fidelity, as demonstrated by scanning electron microscope. PSC cultured on the unpatterned control. (B) hPSC cultured on the unpatterned control exhibited a spread morphology in contrast to the elongated morphology observed on 2 μm gratings. Cells were stained for phalloidin (green) and 4′,6-diamidino-2-phenylindole (blue). Scale bars: 5 μm (left) and 200 μm (right). (C) The filopodia of hPSC on 2 μm gratings extend along, across and between gratings (white arrows). Adapted from Chan et al. (2013).