Figure 2.

Lipin1 Levels Are Upregulated during Development and after Axotomy

(A) Retinal sections from WT mice of different ages (1, 7, 21, and 50 days postnatal) were collected and stained with DAPI (blue), Tuj1 (green), and lipin1 (red). Scale bar: 10 μm.

(B) Percentage of RGCs with a low or high lipin1 level at the indicated ages. ^∗∗p ≤ 0.01, ^∗p ≤ 0.05, ns, not significant, ANOVA followed by Tukey’s test.

(C) Whole-mount retinas from WT mice 3 days after axotomy or sham surgery were collected and stained for DAPI (blue), SMI32 (green), and lipin1 (red). Scale bar: 50 μm. Zoomed-in images are shown in the right panel. Scale bar: 10 μm.

(D) Percentage of αRGCs with a low or high lipin1 level indicated by lipin1 staining. ^∗∗p ≤ 0.01, ANOVA followed by Bonferroni’s test. Error bars indicate SEM.

See also Figure S2.