Figure 3.

Lipin1 Inhibits Axon Regeneration through Its Phosphatidate Phosphatase Activity and Regulates Glycerolipid Metabolism in Neurons

(A) Schematic representation of the different lipin1 overexpression constructs used for the subsequent experiments.

(B) Sections of optic nerves from WT mice at 2 WPI, injected with AAV-lipin1-shRNA combined with AAV-GFP, AAV-lipin1-WT, AAV-lipin1-PAPm, or AAV-lipin1-ΔNLS. Scale bar: 100 μm.

(C) Number of regenerating axons at different distances distal to the lesion site. ^∗∗p ≤ 0.01, ^∗p ≤ 0.05, ANOVA followed by Tukey’s test, n = 5–6 mice.

(D) Heatmap represents the alteration of lipidomes after lipin1 KD in cortical neurons. Lipid species with the top 20 VIP are listed. Colors correspond to differences in relative abundance.

(E and F) Total TG (E) and PC (F) levels in cortical neurons after AAV-control or lipin1-shRNA treatment. ^∗∗p ≤ 0.01, ^∗p ≤ 0.05, Student’s t test. Error bars indicate SEM.

See also Figure S3.