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. 2020 Jan 22;105(2):276–292.e5. doi: 10.1016/j.neuron.2019.10.009

Figure 4.

Figure 4

TG Hydrolysis Is Required for the Axon Regeneration Induced by lipin1 Depletion

(A) Schematic showing the TG metabolism pathway in mammals.

(B) Representative images of DRG neurons cultured with DMSO vehicle, Atglistatin, or KLH-45 for 3 days. BODIPY (green) staining was used to visualize lipid droplet distribution in neurons. Scale bar: 20 μm.

(C) Sections of optic nerves from Rosa26-Cas9 mice with lipin1-sgRNA injection at 2 WPI combined with AAV-control or Atgl shRNA. Scale bar: 100 μm.

(D) Number of regenerating axons at different distances distal to the lesion site. ∗∗p ≤ 0.01, ANOVA followed by Tukey’s test, n = 5–6 mice.

(E) Sections of optic nerves from Rosa26-Cas9 mice with lipin1-sgRNA injection at 2 WPI combined with AAV-control or Ddhd2 shRNA. Scale bar: 100 μm.

(F) Number of regenerating axons at different distances distal to the lesion site. p ≤ 0.05, ANOVA followed by Tukey’s test, n = 5–6 mice. Error bars indicate SEM.

See also Figure S4.