Figure 6.

PL Biosynthesis Is Essential for the Axon Regeneration Induced by lipin1 Depletion

(A) Schematic showing the PL synthesis pathways in mammals.

(B) Representative images of replated neurons from the respective groups with Tuj1 staining. Adult DRG neurons were dissociated and cultured with different AAV shRNA for 10 days. Neurons were then replated and fixed 24 h later. DRG neurites were visualized by Tuj1 staining. Scale bar: 400 μm.

(C) Quantification of the length of the longest axon for each DRG neuron in (B). Three mice and 10–20 cells from each mouse were quantified in each group. ^∗∗p ≤ 0.01, ANOVA followed by Tukey’s test.

(D) Sections of optic nerves from Cas9 mice at 2 WPI. The vitreous body was injected with respective AAVs. Axons were labeled by CTB-FITC. Scale bar: 100 μm.

(E) Number of regenerating axons at the indicated distances distal to the lesion site. ^∗∗p ≤ 0.01, ANOVA followed by Tukey’s test, n = 6 mice.

(F) Sections of optic nerves from WT mice at 2 WPI. The vitreous body was injected with AAV-GFP, Pcyt1a, Pcyt1a-CA, or Pcyt2. Axons were labeled by CTB-FITC. Scale bar: 100 μm.

(G) Number of regenerating axons at the indicated distances distal to the lesion site.

^∗∗p ≤ 0.01, ANOVA followed by Tukey’s test, n = 6 mice. Error bars indicate SEM.

See also Figure S6.