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. 2020 Jan;57:203–216. doi: 10.1016/j.ymben.2019.11.007

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Productivity of model recombinant products in lipid engineered cell pools. cB72.3 and FcFP concentrations in supernatant of batch-cultured CHO-Control^cB72.3, CHO-SCD1^cB72.3 and CHO-SREBF1^cB72.3 pools as well as CHO-Control^FcFP, CHO-SCD1^FcFP and CHO-SREBF1^FcFP pools. These cell pools were generated by stably transfecting host pools engineered to overexpress SCD1 or SREBF1 proteins. The control was constructed using the original pcDNA3.1V5-His/TOPO vector. cB72.3 (A) and FcFP (B) concentrations were measured using an Octet® from supernatants harvested at 48, 96, 144 and 192 h of culture. Specific productivity (C) was calculated for cB72.3 (B) and FcFP (D) using these concentration values and corresponding cell growth measurements from the same time points. (D) shows the relative amount of FcFP mRNA transcript in CHO-SCD1^FcFP cells compared to CHO-Control^FcFP expressing cells from samples collected after 72 h of batch culture relative to the two house keeping genes β-actin or GAPDH. n = 3 for each data point and error bars show ± one standard deviation.