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. 2020 Jan 15;10:2977. doi: 10.3389/fmicb.2019.02977

FIGURE 1.

FIGURE 1

Bacterial species isolated from patients at the Walter Reed National Military Medical Center inhibit Staphylococcus aureus growth. (A) Venn Diagram of anti-S. aureus activity: thirty-four clinical isolates were screened for anti-S. aureus activity in an in vitro bacterial interaction assays against S. aureus strains 2014.N, LAC and Mu50. 28/34 isolates tested possessed anti-S. aureus activity against at least one S. aureus strain. 6/34 possessed no activity against S. aureus. 8/34 inhibited growth of all S. aureus strains tested while the remaining 20 strains showed activity against one or two of the S. aureus strains as indicated in the Venn Diagram. (B) Representative Bacterial Interaction Assays: C. aurimucosum (C. aur-1), M. yunnanensis (M. yun), or S. saprophyticus (S. sap) were spotted onto an agar plate that had been seeded with the indicated S. aureus strains (2014.N, LAC, or Mu50). Plates were incubated at 28°C, and images were taken at 24, 72, and 120 h (24 and 120 h images are shown). Images are representative of three independent biological replicates. Scale bar = 10 mm and is the same in the corresponding 24 h and 120 images; in some cases the 120 h spots appear larger than the 24 h spots due to growth of the bacteria within the spots.