FIGURE 6.

Anti-S. aureus activity is retained in Conditioned Cell Free Medium. S. aureus strains LAC or Mu50 were spread on the BHI agar surface and a sterile disk was placed in the center of the plate. Fifty μl of concentrated CCFM prepared from C. amy-2; (incubated with Mu50), S. sap (incubated with LAC), and S. epi-3 (incubated with LAC) that was Heat-Treated (H+) or maintained at room temperature (H–) prior to use was inoculated onto the disk and allowed to dry. Images of the ZOC were taken after 72 h of incubation. Images are representative of three independent biological replicates. Scale bar = 10 mm.