FIGURE 7.

Staphylococcus aureus-infected Galleria mellonella. Galleria mellonella caterpillars were divided into the indicated groups and were monitored for survival over 120 h at 37°C after the indicated inoculations. (A,B) Untouched and PBS injected Galleria served as negative controls and the indicated doses of 2014.N, LAC and Mu50 were tested. (C) For the treatment experiments, untouched caterpillars were maintained. Additionally, PBS injected caterpillars were treated 1-h post S. aureus infection with CCFM prepared from BHIT in which no bacteria had been cultured to ensure no effect of dual injection on survival. Caterpillars that were injected with 10⁶ CFU of S. aureus strain LAC were treated 1-h post infection with BHIT CCFM (sham treatment) or S. epi-3 or S. sap derived CCFM. Kaplan-Meier survival curves were compared (excluding negative controls) using the Mantel–Cox test with Holm’s correction for multiple comparisons. In (A,B), the three S. aureus strains were compared to identify statistically significant differences in Galleria mortality; untouched and PBS dosed caterpillars were not included in the comparisons. In (A), Mu50 was significantly different than both 2014.N and LAC. In (B), significant differences between the various groups are indicated. In (C), BHIT treated caterpillars were compared to CCFM treated (S. epi-3 or S. sap) Galleria to identify difference; only S. sap was significantly different. Asterisks signifying the P value as follows: ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001; ^∗∗∗∗P < 0.0001.