Table 2.
Quality control result.
| Item | Background average intensity | Internal control gene | Hybridization control | ||
|---|---|---|---|---|---|
| Mean of background intensity (Cy5) < 200 | Mean of IHC intensity > 37500 | ETQC high | ETQC median | ETQC low | |
| Control 1 | 110 | 55446 | 309703 | 71525 | 5099 |
| Control 2 | 106 | 62694 | 295396 | 70600 | 5255 |
| Control 3 | 95 | 77902 | 373575 | 74693 | 5801 |
| Tac 1 | 101 | 48665 | 319491 | 73558 | 5223 |
| Tac 2 | 98 | 46770 | 340991 | 74995 | 5517 |
| Tac 3 | 101 | 61230 | 338713 | 72997 | 5549 |