Skip to main content
. 2019 Dec 19;4(24):e122399. doi: 10.1172/jci.insight.122399

Figure 6. Recombinant MMP-7 decreases nephrin in a time- and dose-dependent manner in ex vivo glomeruli.

Figure 6

(A) MMP-7 treatment (50 nM, 30 min) causes a significant time-dependent release of albumin from isolated rat glomeruli. *P < 0.05, repeated-measures 1-way ANOVA. (B) TEM of isolated glomeruli shows flattening and fusion of foot processes in MMP-7–treated glomeruli compared with vehicle controls (arrows) (original magnification, ×20,000). In separate experiments, glomeruli were incubated with either the same concentration of MMP-7 (50 nM) for various periods of time (C) or with increasing amounts of MMP-7 for 60 minutes (D). Glomerular lysates were immunoblotted with antibodies against nephrin and actin. (E) Nephrin depletion by MMP-7 is dependent on its proteolytic activity. Rat glomeruli were pretreated with GM6001 (25 μM) or MMP Inhibitor II (20 μM) for 30 minutes before incubation with MMP-7 (25 nM or 50 nM) for 60 minutes.