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. 2019 Dec 19;4(24):e122399. doi: 10.1172/jci.insight.122399

Figure 7. MMP-7 reduces nephrin and podocin levels in isolated glomeruli.

Figure 7

(A) Rat glomeruli were incubated separately with MMP-2 (1 μg/ml or 13.88 nM), MMP-7 (50 nM), and MMP-9 (1 μg/ml or 10.9 nM) and glomerular lysates were immunoblotted for nephrin. Only MMP-7 was capable of reducing nephrin levels. (B) MMP-7 also reduced podocin protein in isolated glomeruli ex vivo. Glomerular lysates were immunoblotted with antibodies against podocin and actin. (C) MMP-7 did not affect other membrane proteins in podocytes, such as integrin α3 and β1. Western blot analyses of glomerular lysates reveal no effect on integrin α3 and β1 proteins after incubation with any of the 3 MMPs.