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. 2019 Dec 19;4(24):e130056. doi: 10.1172/jci.insight.130056

Figure 3. Leukotriene released by senescent cells induces profibrotic responses from naive fibroblasts in vitro.

Figure 3

(A) Irradiated human lung fibroblasts (IMR-90) were treated with the ALOX5 inhibitor zileuton (Zi, 50 μM) or the PTGS inhibitor NS-398 (1 μg/mL) for 24 hours prior to the conditioned medium (CM) collection. CM was collected 2 and 20 days after radiation and applied to nonsenescent IMR-90 fibroblasts for 48 hours. (B and C) Total RNA was isolated, reverse transcribed, and analyzed by quantitative PCR. Signal was normalized to tubulin mRNA. The profibrotic responses were assessed by the expression of COL1A2 (B) and α-SMA mRNA (C). Data are presented as mean ± SEM of at least 3 replicates. Statistical analyses were performed using 1-way ANOVA. Asterisk represents the statistical differences calculated by time point. Statistical differences calculated within the same time point group (2 or 20 days); *P ≤ 0.05; **P ≤ 0.01.