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. 2019 Dec 19;4(24):e130056. doi: 10.1172/jci.insight.130056

Figure 5. Inhibition of ALOX5 activity modulates the expression of proinflammatory SASP factors.

Figure 5

Irradiated human lung fibroblasts (IMR-90) were treated with the ALOX5 inhibitor BW-B70C (BW, 10 μM) for 10 days. Total RNA was isolated, reverse transcribed, and analyzed by quantitative PCR. Signal was normalized to tubulin mRNA. (A and B) Heatmap representation of the level of expression mRNA levels of proinflammatory SASP components (A) and the level of expression mRNA levels of profibrotic SASP components (B). (C) Decreased mRNA expression of genes encoding LT receptors in senescent cells 10 days after irradiation. Data are presented as mean ± SEM of at least 3 replicates. Statistical analyses of A and B were performed using 2-way ANOVA, and significance was determined for C by 2-tailed Student’s t tests with Welch’s correction. **P ≤ 0.01; ****P ≤ 0.0001.