Table 6.

Statistical analysis for electrophysiological characteristics

	Kruskal–Wallis	Pairwise comparisons a
		PV+ vs PV+-Dbx1+	PV+ vs Npas1+	PV+ vs Lhx6+	PV+ vs Foxp2+	PV+ vs Dbx1+	Npas1+ vs Lhx6+	Npas1+ vs Foxp2+	Npas1+ vs Dbx1+	Npas1+ vs PV+-Dbx1+	Foxp2+ vs Lhx6+	Lhx6+ vs Dbx1+	Lhx6+ vs PV+-Dbx1+	Foxp2+ vs Dbx1+	Foxp2+ vs PV+-Dbx1+	Dbx1+ vs PV+-Dbx1+	Npas1+-Lhx6+ vs PV+	Npas1+-Lhx6+ vs Npas1+
Spontaneous activity (Hz)	<0.0001	0.24	<0.0001	<0.0001	<0.0001	0.0080	0.066	0.016	0.0070	<0.0001	0.00090	0.11	0.00011	<0.0001	<0.0001	0.011	0.00014	0.29
CVISI	<0.0001	0.21	<0.0001	<0.0001	<0.0001	0.24	0.073	0.067	<0.0001	0.00072	0.0065	0.0070	0.019	<0.0001	<0.0001	0.44	<0.0001	0.43
Max rate (Hz)	<0.0001	0.014	<0.0001	<0.0001	<0.0001	0.074	0.12	0.094	<0.0001	<0.0001	0.010	<0.0001	0.0010	<0.0001	<0.0001	0.24	NA	NA
Max current (pA)	<0.0001	0.0035	<0.0001	<0.0001	<0.0001	0.013	0.054	0.091	<0.0001	0.00011	0.0038	0.00049	0.0050	<0.0001	<0.0001	0.31	NA	NA
Membrane potential (mV)	0.0011	0.012	0.032	<0.0001	0.0021	0.29	0.060	0.13	0.12	0.34	0.43	0.0025	0.15	0.015	0.23	0.062	NA	NA
Trough potential (mV)	<0.0001	0.24	<0.0001	<0.0001	<0.0001	0.0098	0.00086	0.10	<0.0001	<0.0001	0.00024	0.014	<0.0001	<0.0001	<0.0001	0.0078	0.00027	0.0060
Sag ratio	<0.0001	0.19	<0.0001	0.00019	<0.0001	0.00011	0.0034	0.055	0.14	0.00026	0.00019	0.14	0.062	0.011	<0.0001	0.015	0.49	<0.0001
First/last IS ratio	<0.0001	0.0010	<0.0001	<0.0001	<0.0001	0.0087	0.0023	0.22	0.00015	0.0033	0.080	0.092	0.32	0.011	0.056	0.24	NA	NA
AP height (mV)	0.093	0.0046	0.27	0.18	0.33	0.070	0.41	0.19	0.22	0.035	0.13	0.27	0.041	0.058	0.0063	0.14	NA	NA
AP width (ms)	<0.0001	0.025	<0.0001	<0.0001	<0.0001	0.074	0.10	0.24	<0.0001	<0.0001	0.032	0.00021	0.0020	<0.0001	<0.0001	0.30	NA	NA

	Kruskal-Wallis	Npas1+-Lhx6+ vs Foxp2+	Npas1+-Lhx6+ vs Dbx1+	Npas1+-Lhx6+ vs PV+-Dbx1+	Lhx6+bright vs Npas1+	Lhx6+dim vs Npas1+	Lhx6+bright vs PV+	Lhx6+dim vs PV+	Lhx6+bright vs Foxp2+	Lhx6+dim vs Foxp2+	Lhx6+bright vs Dbx1+	Lhx6+dim vs Dbx1+	Lhx6+bright vs PV+-Dbx1+	Lhx6+dim vs PV+-Dbx1+	Lhx6+bright vs Npas1+-Lhx6+	Lhx6+dim vs Npas1+-Lhx6+	Lhx6+bright vs Lhx6+dim
Spontaneous activity (Hz)	<0.0001	0.021	0.092	0.00044	0.44	0.014	<0.0001	0.45	0.14	0.00041	0.0013	0.19	<0.0001	0.17	0.17	0.020	0.0039
CVISI	<0.0001	0.11	0.0049	0.011	0.22	0.060	0.00015	0.54	0.0070	0.011	0.0063	0.79	0.050	1.0	0.51	0.15	0.33
Max rate (Hz)	<0.0001	NA	NA	NA	0.95	0.00048	<0.0001	0.0051	0.040	<0.0001	<0.0001	0.24	<0.0001	0.78	NA	NA	0.00054
Max current (pA)	<0.0001	NA	NA	NA	0.44	0.00034	<0.0001	0.028	0.011	0.00016	<0.0001	0.98	0.00038	0.50	NA	NA	0.0016
Membrane potential (mV)	0.001	NA	NA	NA	0.99	<0.0001	0.072	<0.0001	0.28	0.087	0.18	<0.0001	0.85	<0.0001	NA	NA	<0.0001
Trough potential (mV)	<0.0001	0.00091	0.11	0.00034	0.039	0.00088	<0.0001	0.013	0.00048	0.00082	<0.0001	0.64	<0.0001	0.0088	0.058	0.34	0.010
Sag ratio	<0.0001	<0.0001	0.0033	0.25	0.58	0.025	<0.0001	0.14	0.026	0.0010	0.21	0.17	0.0019	0.69	<0.0001	0.27	0.020
First/last ISI ratio	<0.0001	NA	NA	NA	0.020	0.0058	<0.0001	0.00039	0.27	0.097	0.16	0.31	0.36	0.70	NA	NA	0.62
AP height (mV)	0.093	NA	NA	NA	0.94	0.72	0.76	0.21	0.44	0.26	0.43	0.75	0.13	0.44	NA	NA	0.70
AP width (ms)	<0.0001	NA	NA	NA	0.41	0.0047	<0.0001	0.010	0.044	0.0030	<0.0001	0.16	<0.0001	0.49	NA	NA	0.012

^a Two-tailed p values are listed. The first column represents p-values from Kruskal–Wallis tests comparing each characteristic across all groups, with the Lhx6 neurons all grouped together; the second set of columns give p-values from Dunn tests comparing each pair of neuron types, with the Lhx6 neurons all grouped together; the third set of columns give p-values from Mann–Whitney tests comparing the Lhx6⁺_bright and Lhx6⁺_dim neuron subtypes with all other neuron types. The bolded p-values are significant when considering the Bonferroni-corrected thresholds, which are equivalent to 0.05/10, 0.05/170, and 0.05/118, respectively.

AP, Action potential; CV, coefficient of variation; ISI, interspike interval; MAD, median absolute deviation.