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. 2020 Jan 22;40(4):796–810. doi: 10.1523/JNEUROSCI.0455-19.2019

Figure 2.

Figure 2.

No overt degeneration or remodeling in G2 mouse retinas. A, Similar thickness in the ONL and OPL of 6 month WT and G2 retinas. Top, Representative vertical sections labeled with DAPI. Optic nerve head labeled ON. Thickness measurements were performed in two distinct regions: (1) 500 and (2) 800 μm from optic nerve (white boxes). Bottom, ONL and OPL thickness is not significantly different (N.S.) at 500 μ or 800 μm (two-way RM ANOVA; see Results). n = 4 mice, 3–4 sections/mouse. Scale bar, 100 μm. B–D, Confocal images of immunolabeled retinal sections show normal retinal structures in 6 month WT (B–D), G2 (B′–D′) and G2::Cx36 retinas (B″–D″). B–B″, Calbindin (green) labels horizontal cells (arrow) and some types of amacrine cells (arrowhead). C–C″, Immunolabeling of rod bipolar cells with antibody against PKCα (green), ribbon synapses in photoreceptor terminals with antibody against CtBP2 (red) and cone terminals labeled with PNA (blue). Arrows point to representative extended rod bipolar cell dendrites rarely observed in both WT and G2 retinas. Insets show detailed synaptic structure of boxed regions. D–D″, Immunolabeling of Cx36 gap junctions with antibody against Connexin 36 (Cx36, red) and cone terminals with antibody against cone arrestin (CAR, cyan). Scale bars, 20 μm. E, Amplitudes of dark-adapted flash ERG b-waves as a function of rod photoisomerizations elicited by brief flashes for WT (black circles; n = 10), G2 (red triangles; n = 10), and G2::Cx36 mice (purple diamonds; n = 10) at 3 months of age. G2 and G2::Cx36 mice exhibit normal rod-driven flash ERG responses and reduced cone-driven flash ERG responses. Statistical analysis: two-way RM ANOVA; see text for details. Error bars: SEM is indicated but generally smaller than the size of the symbols. F, b-wave amplitudes of WT, G2, and G2::Cx36 mice measured at 3 and 6 months of age. Minor changes in amplitude suggest no significant degeneration or remodeling during the time course of experiments. Statistical analysis: two-way RM ANOVA, *p < 0.05; see text for details.