Figure 3.

The distance between the soma and first branch point of L2/3 pyramidal neuron apical dendrites is normal in C1qa^−/− mice. A, Quantification of the correlation between spine numbers and distance between soma and first branch point in P10 (left), P20 (middle), and P29–P30 (right) animals, analyzed per cell. The spine numbers and distance are significantly correlated at the two older ages, using Spearman's rank correlation coefficient r_s (P10 WT: r_s = 0.2616, p = 0.3081, n = 17 cells; P10 C1qa^−/−: r_s = 0.5366, p = 0.0047, n = 26 cells; P20 WT: r_s = 0.6199, p = 0.0003, n = 30 cells; P20 C1qa^−/−: r_s = 0.5634, p = 0.0041, n = 24 cells; P29–P30 WT: r_s = 0.7321, p = 0.0001, n = 22 cells; P29–P30 C1qa^−/−: r_s = 0.7085, p < 0.0001, n = 26 cells). B, Quantification of the distance between the soma and the first branch point in P10 (left), P20 (middle), and P29–P30 (right) animals. This distance is not different between WT and C1qa^−/− animals at any of the ages (P10: unpaired two-tailed t test, t₍₇₎ = 0.2727, p = 0.7929, n = 4–5 animals/genotype; P20: unpaired two-tailed t test, t₍₈₎ = 0.5198, p = 0.6173, n = 4–6 animals/genotype; P29–P30: unpaired two-tailed t test, t₍₁₀₎ = 0.3156, p = 0.7588, n = 6 animals/genotype). All error bars represent SEM. ns, not significant.