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. 2019 Jul 1;19(8):5524–5529. doi: 10.1021/acs.nanolett.9b02062

Figure 3.

Figure 3

SMFS experiments on VHH(WT) and disulfide bond knockout mutants C24A, C98A, and C24A/C98A. (a) Dynamic force spectra of VHH library members obtained from four pulling speeds from 400 to 3, 200 nm/s. Points shown represent the most probable rupture force versus average loading rate at a given pulling speed. The dashed line represents the Bell-Evans model fit. (b) Rupture force histograms obtained at each pulling speed. (c) Data fitting with the DHS model was used to extract energy landscape parameters shown in Table 2.