Table 2. Most Probable Rupture Forces (Errors Are Full Width at Half-Maximum for Each Rupture Force Distribution) Obtained Different Pulling Speeds and Energy Landscape Parameters (±SD) Based on the DHS Model (v = 0.5).
| most
probable rupture force (pN) |
Dudko–Hummer–Szabo
model |
||||||
|---|---|---|---|---|---|---|---|
| sample | 400 nm/s | 800 nm/s | 1600 nm/s | 3200 nm/s | ΔG (kBT) | Δx (nm) | t0 (s) |
| VHH_WT | 68.78 ± 9.85 | 70.21 ± 10.15 | 73.04 ± 12.29 | 73.42 ± 15.65 | 10.91 ± 1.57 | 0.61 ± 0.16 | 11.02 ± 7.93 |
| C24A | 67.18 ± 14.12 | 69.42 ± 15.33 | 72.72 ± 19.63 | 76.44 ± 22.55 | 10.47 ± 3.01 | 0.59 ± 0.20 | 12.16 ± 8.91 |
| C98A | 65.67 ± 16.07 | 69.82 ± 19.01 | 71.6 ± 22.71 | 75.46 ± 25.16 | 9.22 ± 1.96 | 0.49 ± 0.21 | 3.06 ± 2.87 |
| C24A/C98A | 59.11 ± 12.76 | 64.07 ± 14.65 | 69.06 ± 19.13 | 71.42 ± 20.54 | 8.93 ± 1.93 | 0.46 ± 0.20 | 2.12 ± 2.01 |