. 2019 Dec 23;8:e51163. doi: 10.7554/eLife.51163

Table 1. Estimates of K_m and k_cat for Substrate Ubiquitylation.

Substrate	Chain modifying E2 or E3	E3	K_M (10⁻⁹ M)	k_cat (min⁻¹)	R²
Cyclin E	ARIH1	SCF^FBW7	149 ± 36	0.29 ± 0.02	0.90
β-Catenin	ARIH1	SCF^βTRCP	486 ± 138	0.32 ± 0.03	0.87
Cyclin E	UBE2D3	SCF^FBW7	1673 ± 460	0.77 ± 0.06	0.89
β-Catenin	UBE2D3	SCF^βTRCP	488 ± 53	1.9 ± 0.04	0.97
Cyclin E	UBE2R2	SCF^FBW7	ND	ND
β-Catenin	UBE2R2	SCF^βTRCP	*1900 ± 100
Ub-Cyclin E	UBE2R2	SCF^FBW7	317 ± 44	3.7 ± 0.1	0.96
Ub-β-Catenin	UBE2R2	SCF^βTRCP	292 ± 35	3.2 ± 0.1	0.97
Ub-Cyclin E	UBE2G1	SCF^FBW7	1300 ± 20	2.3 ± 0.1	0.96

^*(Hill et al., 2018); ND, not determined.

Table 1. Estimates of Km and kcat for Substrate Ubiquitylation.