Table 3. Estimates of the rates of ubiquitin transfer to substrate determined as kobs values (sec−1).
Table 3—source data 1. P-values for the fit of the pre-steady ubiquitylation reaction data to the model.
elife-51163-table3-data1.docx (15.3KB, docx)
| Substrate prior to ubiquitin transfer* | |||||
|---|---|---|---|---|---|
| Substrate | Chain modifying E2 or E3 | E3 | S0 | S1 | S2 |
| Cyclin E | ARIH1 | SCFFBW7 | 0.5 ± 0.04 (0.2 ± 0.005) | 0.2 ± 0.03 (0.1 ± 0.01) | 0.08 ± 0.01 (0.1 ± 0.02) |
| β-Catenin | ARIH1 | SCFβTRCP | 0.2 ± 0.01 (0.1 ± 0.005) | 0.3 ± 0.07 (0.2 ± 0.02) | ND (0.1 ± 0.02) |
| Cyclin E | UBE2D3 | SCFFBW7 | 0.1 ± 0.01 (0.06 ± 0.003) | 0.2 ± 0.08 (0.2 ± 0.03) | ND (ND) |
| β-Catenin | UBE2D3 | SCFβTrCP | 5 ± 0.6 (3 ± 0.2) | 0.2 ± 0.04 (0.2 ± 0.03) | ND (0.5 ± 0.1) |
| Cyclin E | UBE2R2 | SCFFBW7 | 0.2 ± 0.02 (ND) | 40 ± 4 (ND) | 4 ± 0.5 (ND) |
| β-Catenin | UBE2R2 | SCFβTrCP | 0.1 ± 0.01 (ND) | 30 ± 3 (ND) | 7 ± 0.5 (ND) |
| Cyclin E | ARIH1/UBE2R2 | SCFFBW7 | 0.2 ± 0.06 (0.2 ± 0.006) | ND (0.4 ± 0.03) | ND (1 ± 0.1) |
| β-Catenin | ARIH1/UBE2R2 | SCFβTrCP | 0.3 ± 0.05 (0.07 ± 0.006) | 2 ± 0.6 (0.6 ± 0.08) | 5 ± 0.8 (1 ± 0.1) |
| Cyclin E | UBE2D3/UBE2R2 | SCFFBW7 | 0.2 ± 0.01 (0.1 ± 0.006) | 7 ± 1 (3 ± 0.4) | 4 ± 0.3 (2 ± 0.1) |
| β-Catenin | UBE2D3/UBE2R2 | SCFβTrCP | 4 ± 0.5 (3 ± 0.3) | 0.9 ± 0.1 (0.4 ± 0.04) | 4 ± 0.4 (1 ± 0.1) |
| Ub-Cyclin E | UBE2G1 | SCFFBW7 | ND (ND) | 1.0 ± 0.1 (ND) | 1.0 ± 0.1 (ND) |
*Rates are given for both saturating E2/E3 for SCF or for estimated cellular E2/E3 for SCF (in parenthesis and italics). P-values for the goodness of fit to the model are provided in Table 3—source data 1.
ND; not determined or not statistically significant.