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. 2020 Jan 22;6(4):eaax6646. doi: 10.1126/sciadv.aax6646

Fig. 4. MRIgFUS delivery of D3 increased TrkA-dependent signaling cascades in the basal forebrain 90 min after treatment.

Fig. 4

(A) Representative Western blots from the MS/DBB and NBM after PBS, PBS/FUS, D3, or D3/FUS treatment. Blots were probed for pTrkA, pAkt, pMAPK, pCREB, pJNK, the corresponding total proteins, and βtubIII to control for loading. Levels of (B) pTrkA, (C) pAkt, (D) pMAPK, and (E) pCREB were reduced in PBS-treated TgCRND8 mice relative to non-Tg mice and stimulated in D3/FUS-treated mice compared to PBS- and D3-treated (no MRIgFUS) controls in the MS/DBB and NBM for both genotypes. (F) Elevated levels of pJNK were found in control TgCRND8 mice relative to non-Tg mice. pJNK was decreased in TgCRND8 mice after D3/FUS treatment compared to control conditions in Tg mice, but levels remained unaltered in non-Tg mice. Statistics: Two-way ANOVA. Significance: *,†P < 0.05; **,††P < 0.01, ***,^^^P < 0.001; † indicates comparison with PBS-treated non-Tg mice (genotype effect); ^ indicates comparison with PBS-treated mice of the same genotype (FUS effect); * indicates comparison with D3-treated group (i.e., intravenous D3, no MRIgFUS) of the same genotype (D3/FUS effect). Data represent means + SEM; n = 6 per group.