Table 1. Kinetic constants for WT human ABHD14B against different acylated esters of pNp.
Substrate | kcat (min-1) | Km (mM) | kcat/Km (M-1s-1) |
---|---|---|---|
pNp-acetate | 2.2 ± 0.8 | 1.8 ± 0.6 | 20.4 ± 2.5 |
pNp-butyrate | 0.6 ± 0.2 | 3.1 ± 0.6 | 3.2 ± 0.4 |
pNp-octanoate | 0.2 ± 0.1 | 3.9 ± 0.7 | 0.9 ± 0.2 |