Circ-ZKSCAN1 inhibition retards NSCLC cells’ biological process. (A) Relative circ-ZKSCAN1 expression in A549 and H1299 cells after transfection with si-circ-ZKSCAN1 or control. CCK-8 assays (B) and colony formation assays (C,D,E) in A549 and H1299 cells treated with or without circ-ZKSCAN1 inhibition. (F,G,H) A549 and H1299 cells in transwell chambers were applied to cell migration assays (100×). (I,J,K) Cells treated with si-NC or si-circ-ZKSCAN1 to conduct wound healing assays. (L,M,N) Flow cytometry to determine cell apoptosis of A549 and H1299 after si-circ-ZKSCAN1 or si-NC. (**P<0.01, ***P<0.001). NSCLC, non-small cell lung cancer.