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. 2019 Sep 25;39(4):801–813. doi: 10.1038/s41388-019-1010-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — PKR affects the distribution and release of misfolded PrPs. a Our experimental model. b Immunofluorescent confocal microscopic analysis of native PrP expression in HeLa and HeLaPKRkd cells 48 h after transfection with Ad-Prion (2500 viral particles/cell) or without transfection. HeLaPKRkd cells exhibited excess surface PrPs (mostly in misfolded form) after Ad-Prion transfection. c Average levels of native and misfolded PrP expression in HeLa and HeLaPKRkd cells 48 h after transfection with Ad-Prion. Native and misfolded PrP values were normalized according to isotype control values. Experiments were performed in triplicate; data are presented as means. d Western blot of HeLa and HeLaPKRkd cells for expression of PKR, p-PKR, and PrPs 48 h after transfection with Ad-Prion (2500 viral particles/cell) or without transfection. Actin was used as a loading control. e Immunofluorescent confocal microscopic images of native PrP expression in HeLaPKRkd cells and HeLaPKRkd cells with re-expression of PKR (or Luc) 48 h after transfection with Ad-Prion (2500 viral particles/cell) or without transfection. f Western blot analysis of native PrPs in HeLaPKRkd cells with re-expression of PKR (or Luc) 48 h after transfection with Ad-Prion (2500 viral particles/cell) or without transfection