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. 2019 Sep 27;39(4):849–861. doi: 10.1038/s41388-019-1027-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Histology of WT spheroids and corresponding tumor material. Representative spheroids are depicted by low power micrographs (brightfield, row 1). H&E staining showed similar histology of tumor material and corresponding spheroids (rows 2 and 3). IHC for renal progenitor/blastemal markers (SIX1/2, NCAM, and PAX2), the mesenchymal marker vimentin (VIM), the epithelial marker cytokeratin 7/8 (Cam5.2, CK7/8), and the proliferation marker Ki-67 was done on FFPE sections of spheroids. Scale bar: bright field 250 µm, H&E and IHC 25 µm