Fig. 3.

Spheroid plasticity upon adherent cultivation. a Spheroids dissociated into single cell suspensions could be grown adherently on cell culture grade plates (S>A). While blastemal cells quickly lose proliferation capacity (WT01 shown as an example), epithelial, and stromal derived cells could be passaged more than ten times and retained spheroid forming capacity for three passages (S>A>S). p passage. Scale bar: 200 µm. b Quantitative RT-PCR of SIX2, PAX2, TGFB2, and CTGF in spheroid (S), spheroid derived adherent (S>A), and corresponding primary adherent (A) cultures. For WT04 and WT05 early (S>A_p1) and later passages (S>A_p4/p5), not capable of forming spheroids, were used. Blastemal spheroid culture WT01-S was taken as a reference to calculate ΔΔct-values