Figure 2.

ELP fusion improves NS1-ER accumulation levels. N. benthamiana tissue was infiltrated with two different constructs, and the proteins were extracted with PEB buffer. Each lane was loaded with 20 μg of TSP from three replicates and was detected with anti c-Myc antibody. NS1 accumulation results represent the average of three biological replicates consisting of three different plants. Expression levels were measured by densitometry analysis using known amounts of c-Myc–tagged CBD protein (12.5–200 ng). Error bars are standard error of the mean (SEM). ^∗∗Statistically significant difference by Student's t-test (p < 0.01).