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Triton X-100 improves extraction of NS1 from the ER. N. benthamiana leaves were infiltrated with seven constructs targeting the protein to different subcellular compartments. Proteins were extracted using two different buffers containing non-ionic detergents: 0.1% (v/v) Tween 20 (1) and 1.5% (v/v) Triton X-100 (2), the same volume of TSP (20μl/lane) was loaded per lane. The NS1 HFBI-ER construct showed protein accumulation only when extracted with Triton X-100 buffer.
