Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 16;10:1674. doi: 10.3389/fpls.2019.01674

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Marques, Silva, Dutra, Florean, Menassa and Guedes

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Plant produced NS1 as antigen for dengue diagnosis. (A) Recognition of NS1 ELP-ER produced in N. benthamiana by DENV anti-NS1 monoclonal antibody. NS1 ELP-ER was analyzed under non-reducing conditions. Immunoblotting with anti-NS1 monoclonal antibody against a conformational epitope revealed multimeric bands. p19-infiltrated leaves were used as negative control. (B) IgM–ELISA of the sera from 27 dengue positive patients against total soluble proteins from NS1 ELP-ER leaves or p19-infiltrated control (p19). The same samples were tested against each extract to evaluate the specificity of plant-produced NS1 ELP-ER. The means were significantly different as calculated by paired t-test, p < 0.0003. (C) NS1-ELP ELISA for IgM detection in dengue positive (n = 27) and negative (n = 27) sera. Unpaired t-test p < 0.0001. Mean value is shown by the thin line and the standard error of the mean (SEM) is represented by “whiskers.”.