Fig. 4.

Bioactivity is caused by ammonia in a glycine cleavage-dependent manner. a Volatile activity and ammonia production by S. griseus, S. griseus glycine cleavage mutant gcvP (ΔUTR-P), S. griseus glycine cleavage mutant gcvT (ΔUTR-T), S. griseus glycine cleavage double mutant gcvT-gcvP (ΔUTR-T/ΔUTR-P), S. coelicolor, and the S. coelicolor glycine cleavage system mutant ΔgcvP. b Scheme representation of the reactions carried by the glycine cleavage (GCV) system, consisting of pyridoxal phosphate-containing glycine decarboxylase GcvP (blue); THF-dependent aminomethyltransferase GcvT (yellow); dihydrolipoamide dehydrogenase GcvL; and lipoic acid-containing carrier protein GcvH. c Illustration of the induction of volatile antibiosis in different Streptomyces strains when increasing concentrations of glycine are added