Table 2.
Validation of optimum MAE condition for alkaloids and polyphenolic antioxidants in B. jaeschkeana roots and tested the same on B. asiatica roots.
| Dependent Variables | Berberis jaeschkeana | Berberis asiatica | ||
|---|---|---|---|---|
| Predicted value | Experimental Value | CV (%) | Experimental value | |
| Berberine (mg/g dw) | 49.14 | 46.38 | 5.61 | 88.71 |
| Palmatine (mg/g dw) | 21.47 | 20.54 | 4.33 | 18.68 |
| TP (mg GAE/g dw) | 21.85 | 21.27 | 2.65 | 30.43 |
| ABTS (mM AAE/g dw) | 21.72 | 21.96 | 1.09 | 26.47 |
| FRAP (mM AAE/g dw) | 231.30 | 230.14 | 0.50 | 247.37 |
| DPPH (mM AAE/g dw) | 52.89 | 53.73 | 1.56 | 66.21 |
TP = total phenols, ABTS = 2, 2′- azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical cation inhibition, FRAP = Ferric reducing antioxidant power, DPPH = 2, 2-diphenyl-1-picrylhydrazyl radical scavenging ability, AAE = ascorbic acid equivalent; GAE = gallic acid equivalent; dw = dry weight, CV = coefficient of variation.