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. 2020 Jan 22;10:939. doi: 10.1038/s41598-020-57777-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental setup in the hind leg model of the rat. Flowmeter, simultaneous laser-Doppler flowmetry and tissue spectrophotometry and indocyanine green-videoangiography with integrated FLOW 800 tool were used to detect different degrees of stenotic situations at the A. and V. femoralis (0%, 25%, 50%, 75% and 100%) and analyse the consecutive blood flow changes.