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. 2020 Jan 22;10:933. doi: 10.1038/s41598-020-57805-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tempo-spatial expression patterns of CsTPS genes in tea apical buds (B), young leaves (YL), mature leaves (ML), old leaves (OL), immature stems (S), flowers (FL), young fruits (FR) and roots (R). (A), CsTPS genes substantially expressed in eight tested organs with a few exceptions; (B), CsTPS genes predominantly expressed in leaves; (C), CsTPS genes primarily expressed in flowers; Transcripts per million (TPM) were used to evaluate gene expression levels; (D) qRT-PCR validation of transcriptomic data of the CsTPS genes in red in young and mature leaves (YL and ML) and flowers at the two developmental stages (F1 and F2) relative to the level of CsTPS02 in F1. Mean levels (n ≥ 3) distinguished with different letters for each gene among different leaves and flowers are significantly different from each other (p < 0.05). Sesqui-syn, Mono-syn, and Bifunctional-syn represent sesquiterpenoid synthase, monoterpenoid synthase and bifunctional ternpenoid synthase, respectively.