Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 22;10:1004. doi: 10.1038/s41598-020-57989-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

EMR2 and CD97 stimulate G protein coupling in a yeast-based reporter assay. Constructs expressing full-length (-FL) or truncated (-CTF) forms of EMR2 (a) and CD97 (b) were transformed into yeast strains expressing yeast Gpa1p or chimeric Gpa1/G protein alpha subunits corresponding to mammalian G_α sequences as designated on the x axis. Fluorescence Intensity Units (ex. 485 nm/em. 535 nm) report receptor-stimulated cell growth leading to the production of fluorescein. Data are presented as fold increase over a vector control. (c) Responses induced by EMR2-FL and EMR2-CTF were compared with human somatostatin receptor unstimulated (sst₂) or stimulated (sst₂ + SRIF) with a saturating concentration (1 μM) of somatostatin (SRIF-14). All data are plotted as the mean + range of four independent isolates, two replicates of each.