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. 2020 Jan 22;10:1004. doi: 10.1038/s41598-020-57989-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Activation of Inositol phosphate production by EMR2. EMR2-FL, EMR2-CTF and vector control constructs were transfected into HEK 293 T cells in combination with different G protein alpha subunits, as indicated, and tested in the IP-One HTRF assay (Cisbio). A reduction in the ratio (665 nm/615 nm) indicates an accumulation of inositol monophosphate, a downstream metabolite of IP₃ induced by activation of a phospholipase C cascade. Data represent mean ± SD of 16 replicates for each condition. Statistical significance was measured using unpaired t-test (*p < 0.05).