Figure 10.

The ligation of preinserted 3′-rC and 3′-rA opposite dG by DNA ligase IV/XRCC4 complex. (A) Lanes 1 and 8 are the minus enzyme controls for the nicked DNA substrates with 3′-rC:dG and 3′-rA:dG, respectively. Lanes 2–7 and 9–14 are the ligation products in the presence of Mn²⁺ and correspond to time points of 0.5, 1, 3, 5, 8, and 10 min. (B) Graphs show time-dependent changes in the products of ligation (blue for 3′-rA:dG and green for 3′-rC:dG) and ligation failure (red). The data represent the average of three independent experiments ± SD. Corresponding uncropped gel image is shown in Supplementary Fig. 24.