Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 22;10:953. doi: 10.1038/s41598-020-57587-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Sulfite reductase activity and protein levels of wild-type, mutant, and complemented mutant cell extracts. Upper panel. Sulfite reductase activity was indicated by bands of clearing. No activity was observed in extracts from ΔsirA, ΔsirEF, ΔccmI or ΔccmIΔsirG. Reduced activity was observed in cell extracts from ΔsirH and ΔsirG. Complementation restored reductase activity to wildtype levels, except with ΔccmI, which partially restored activity. Lower panel. Western blot analysis of the cell extracts was performed using antibodies against SirA. Reactive bands that corresponded to SirA are indicated. Reduced SirA was detected in extracts from ΔsirH and ΔsirG compared to wildtype and was absent in extracts from ΔsirA, ΔsirEF, ΔccmI and ΔccmIΔsirG. Complementation restored SirA levels similar to that of the wildtype or corresponding single mutants, in the case of the ΔsirGΔccmI mutant. Full-length blots/gels are presented in Supplementary Fig. S5.