Table 1.
List of strains and plasmids used in this study.
| Strain | Description | Source |
|---|---|---|
| MR-1 | Lake Oneida S. oneidensis isolate | Venkateswaran et al.60 |
| SR1207 | MR-1 ∆SO_0479 (sirA) | Shirodkar et al.10 |
| ∆nrfA | MR-1 ∆nrfA | This Work |
| SR1518 | ∆sirA containing pTC1 | This Work |
| SR1550 | MR-1 ∆SO_0265 (ccmI) | This Work |
| SR1576 | ∆ccmI containing pTC2 | This Work |
| SR1566 | MR-1 ∆SO_0476 (sirH) | This Work |
| SR1570 | ∆sirH containing pTC3 | This Work |
| SR1542 | MR-1 ∆SO_0477-8 (sirEF) | This Work |
| SR1548 | ∆sirEF containing pTC4 | This Work |
| SR1593 | MR-1 ∆SO_0482 (sirG) | This Work |
| SR1592 | ∆sirG containing pTC5 | This Work |
| SR1594 | ∆ccmI ∆SO_0482 (sirG) | This Work |
| SR1595 | ∆ccmI∆sirG containing pTC2 | This Work |
| SR1596 | ∆ccmI∆sirG containing pTC5 | This Work |
| EC100D+ | E. coli EC100 derivative, pir+ | Epicenter Technologies |
| β2155 | pir::RP4, KmR | Dehio et al.38 |
| pER21 | R6K ori, GmR, sacB, lacZ α-fragment | Shirodkar et al.10 |
| pJBC1 | Cloning and sequencing vector, CmR | This Work |
| pTRG | Bacterial two-hybrid target plasmid | Agilent Technologies |
| pBT | Bacterial two-hybrid bait plasmid | Agilent Technologies |
| pTC1 | SO_0479N589C in pJBC1 | This Work |
| pTC2 | SO_0265 (ccmI) in pJBC1 | This Work |
| pTC3 | SO_0476 (sirH) in pJBC1 | This Work |
| pTC4 | SO_0477-8 (sirEF) in pJBC1 | This Work |
| pTC5 | SO_0482 (sirG) in pJBC1 | This Work |
| pBTH1 | SO_0479 (sirA) in pTRG | This Work |
| pBTH2 | SO_0479 (sirA) in pBT | This Work |
| pBTH3 | SO_0482 (sirG) in pTRG | This Work |
| pBTH4 | SO_0482 (sirG) in pBT | This Work |
| pBTH5 | SO_0265 (ccmI) in pTRG | This Work |
| pBTH6 | SO_0265 (ccmI) in pBT | This Work |