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. 2020 Jan 22;10:916. doi: 10.1038/s41598-020-57473-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Variance at low copy number is a property of LAMP. Output fluorescent or bioluminescent results for DNA template dilution series (i), no template control in red, calculated average of 1 copy per partition in grey, 10 copies in orange, 10² copies in light blue, 10³ copies in green, 10⁴ in dark blue, 10⁵ in purple and 10⁶ in pink. Average Ct or Tmax against DNA template concentration (logarithmic scale) (ii) with trendline and error bars. (A) LAMP amplification with 35Sp primers, artificial template and detection with BART; (B) LAMP amplification with NOSt primers, artificial template and BART detection; (C) LAMP amplification with 35Sp primers, artificial template and SYTO9 dye detection; (D) LAMP amplification with 35Sp primers, linearised plasmid and JOE-FIP quenched fluorescence detection; (E) LAMP with ADH1 primers, genomic DNA template and SYTO9 detection; (F) PCR with 35Sp primers, genomic template and SYBR green detection.