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. 2020 Jan 22;10:916. doi: 10.1038/s41598-020-57473-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Reproducibility of results with linearised plasmid template. 35Sp LAMP amplification with SYTO9 detection of 1 to 10 copies of linearised plasmid DNA in blue with additional repeat data for 1 to 5 copies in red. Variance for 120 minute assay time and truncated to 90, 40 and 30 minutes with trendline for 1 to 10 copies (dotted line). Full assay time of 120 minutes (A), truncated to 90 minutes (B), 40 minutes (C) and 30 minutes (D).