Table 1.
Sequence information for artificial 35Sp and NOSt LAMP templates.
Target, Type, Notation | Template Sequence (5’ to 3’) |
---|---|
35Sp, LAMP, BIP Dumbbell | CCACGTCTTCAAAGCAAGTGGGGATAGTGGGATTGTGCGTCCCCTTACGTCAGTGGACCACTTGCTTTGAAGACGTGGTCTAGATTCCACGATGCTCCTCGGGGGTCCATCTTTGGGCCACTGTCGGCAGAGGCGAGGAGCATCGTGGAA |
NOSt, LAMP, FIP Dumbell | CATGCTTAACGTTAATTCAACATGAATCCTGTTGCCGGTCTTGCGATGATTATCATATAATTTTGTTGAATTAACGTTAAGCATGTCTAGAGCATGACGTTATTTATGAGATGATTAGAGTCCCGCAATTATACTAGAAAACAAAATATAGCGCGATCTCATAAATAACGTCATGC |
35Sp and NOSt templates synthesised and PAGE purified by IDT (Coralville, Iowa, USA). Both oligonucleotides were diluted to 10 micromolar and stored at minus 20 degrees C. The 35Sp oligonucleotide is 150 bases and the NOSt is 176 bases.