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LAMP quenched fluorescent primers Modified 35S promoter and NOS terminator FIP primers with JOE (6-carboxy-4’,5’-dichloro-2’,7’-dimethoxyfluorescein) attached to the 5’ end13.
| Target, Type, Notation | Lgth | Primer Sequence (5’ to 3’) |
|---|---|---|
| 35Sp, LAMP, JOE-FIP | 45 | [JOE]CCACGTCTTCAAAGCAAGTGG-TTTT-GGATAGTGGGATTGTGCGTC |
| NOSt, LAMP, JOE-FIP | 46 | [JOE]GCATGACGTTATTTATGAGA-TTTT-TCGCGCTATATTTTGTTTTCTA |
The quenched fluorescent LAMP primer was synthesised and HPLC purified by Sigma Aldrich (Poole, UK).
