Skip to main content
. 2020 Jan 22;11(1):44. doi: 10.1038/s41419-020-2243-4

Fig. 3. TNAP inhibition improved cardiac function and attenuated pathological remodeling post MI in SD rats.

Fig. 3

a Time axis of SD rats. Tetra or saline was administrated to rats from 7 days before operation till sampling (14 days after operation). Echocardiography was performed 13 days after operation. b Heart TNAP activity was assessed by alkaline phosphatase assay kit. c Western blotting was performed to analyze heart TNAP expression. The inactivated TNAP bands were around 57 KD. Statistical result using relative gray values (compared with sham) of the blotting was below the band. d In-gel TNAP activity was assessed by BCIP/NBT method. The activated TNAP was around 130 KD. e Sirus red staining of whole heart fibrosis (bar 1 mm) and LV fibrosis (bar 50 μm). Statistical analyses of fibrotic area were shown below (n = 8, each). e Doppler echocardiography was used to assess hemodynamic and anatomic changes among sham, MI + Saline, and MI + Tetra groups. ***P < 0.001 vs. Sham. ### P < 0.001 vs. MI + Saline (n = 8, each). Abbreviations: EF ejection fraction, FS fractional shortening, IVS interventricular septal thickness at end systole, LVID left ventricular end systolic internal diameter.