Fig. 5.

SERM-induced SIRT1 upregulation is regulated by eNOS

A: Animal experiment protocol

B: Representative images of SA-β gal staining of senescent cells (blue) in the aortas of OVX + SERM mice and OVX + SERM + L-NAME mice are shown in the left panel. There were no differences in SA-β gal positive staining in the two groups.

C: Representative images of Oil Red O-stained cross-sections of the aortic root of OVX + SERM mice and OVX + SERM + L-NAME mice are shown in the upper panel. There were no differences in atherosclerotic lesions in the two groups. Scale bar = 200 µm

D: Representative band of western blot analysis and densitometric analysis is shown in the left panel. There were no differences in SIRT1, Ac-p53, p21, and PAI-1 expression in the two groups.

E: BZA, a SERM, was administrated to eNOS-KO OVX mice. Representative band of western blot analysis and densitometric analysis is shown in the left panel. There were no differences in SIRT1, Ac-p53, p21, and PAI-1 expression between control vehicle and SERM mice.

All data are shown as the mean ± S.D.; n = 3 in each group